A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products

A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products

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Sensitive and point-of-care detection of small toxic molecules plays a key role in food safety.Aflatoxin, a typical small toxic molecule, can cause serious healthcare and economic issues, thereby promoting the development of sensitive and point-of-care detection.Although ELISA is one of the official detection methods, it cannot fill the gap between sensitivity and point-of-care application because it requires a large-scale microplate reader.To employ portable readers in food safety, Pt-catalysis has Typewriter attracted increasing attention due to its portability and reliability.In this study, we developed a sensitive point-of-care aflatoxin detection (POCAD) method via a portable handheld barometer.

We synthesized and characterized Au@PtNPs and Au@PtNPs conjugated with a second antibody (Au@PtNPs-IgG).A competitive immunoassay was established based on the homemade monoclonal antibody against aflatoxins.Au@PtNPs-IgG was used to catalyze the production of O2 from H2O2 in a sealed vessel.The pressure of O2 was then recorded by a handheld barometer.The aflatoxin concentration was inversely proportional to the pressure recorded via the barometer reading.

After optimization, a limit of detection of 0.03 ng/mL and a linear range from 0.09 to 16.0 ng/mL were achieved.Recovery was recorded as 83.

1%−112.0% along with satisfactory results regarding inner- and inter-assay precision (relative standard deviation, RSD < 6.4%).Little cross-reaction was observed.Additionally, the POCAD was validated by high-performance liquid chromatography (HPLC) by using peanut and corn samples.

The portable POCAD exhibits strong potential for applications in the on-site detection of small toxic molecules to ensure Incontinence/Single Use food safety.